• Welcome to

    Lab of Synthetic Proteins

  • ABOUT US

    Research in our group focuses on chemical protein synthesis and its applications to biomedical studies. Students will have good opportunities to participate in interdisciplinary research in the area of protein chemical synthesis and its biomedical applications. Welcome to join us : )

  • RESEARCH

    Proteins are fascinating molecules of life, but not every protein can be obtained biologically. Synthetic chemistry provides a supplementary approach for the production of proteins. This method expands our exploration of chemistry of life and drug development. Our research projects include:

    • Methods of chemical protein synthesis
    • Post-translational modification
    • Mirror-image proteins
    • Protein and peptide drugs

  • PERSONNEL

    lei liu, Tsinghua University

    1995 ~ 1999: B.S. , University of Science and Technology of China

    1999 ~ 2004: Ph.D. , Columbia University

    (with Professor Ronald Breslow)

    2004 ~ 2007: Research Associate, The Scripps Research Institute

    (with Professor Chi-Huey Wong)

     

    2007 ~ present: Professor of Chemistry, Tsinghua University

    2014 ~ present: Vice Chair, Department of Chemistry

    2015 ~ present:Associate Editor, Organic & Biomolecular Chemistry

  • Group Members

    lei liu,Professor of Chemistry, Tsinghua University

    Haiyan Li

    Lab Manager

    hxlihaiyan@mail.tsinghua.edu.cn

    lei liu,Professor of Chemistry, Tsinghua University

    Jiabing Li

    Postdoctoral Researcher

    lxjhbr@gmail.com

    lei liu,Professor of Chemistry, Tsinghua University

    Man Pan

    Graduate Student

    panman.tsinghua@gmail.com

    lei liu, Tsinghua

    Qiaoqiao He

    Graduate Student

    heqq0105@gmail.com

    lei liu, Tsinghua

    Jiaxing Wang

    Graduate Student

    wjxpda@gmail.com

    lei liu, Tsinghua

    Yunkun Qi

    Graduate Student

    qiyunkun@163.com

    lei liu, Tsinghua

    Yanyan Si

    Graduate Student

    yysi2009@gmail.com

    lei liu, Tsinghua

    Shuai Gao

    Graduate Student

    gaoshuai812@gmail.com

    lei liu, Tsinghua

    Xiaodan Tan

    Graduate Student

    skyjusttan@gmail.com

    lei liu, Tsinghua

    Chao Zuo

    Graduate Student

    zuoc14@mails.tsinghua.edu.cn

    lei liu, Tsinghua

    Xianglong Tan

    Graduate Student

    yanzhuzhilong@gmail.com

    lei liu, Tsinghua

    Lujun Liang

    Graduate Student

    lianglj15@mails.tsinghua.edu.cn

    lei liu, Tsinghua

    Lining Lu

    Graduate Student

    lln15@mails.tsinghua.edu.cn

    lei liu, Tsinghua

    Qingyun Zheng

    Graduate Student

    zhengqytsinghua@163.com

  • Alumni

    lei liu, tsinghua university

    Yitong Li

    Graduate Student

    2011-2016

    lei liu, tsinghua university

    Yichao Huang

    Graduate Student

    2011-2016

    lei liu,tsinghua university

    Shan Tang

    Graduate Student

    2011-2016

    lei liu,tsinghua

    Jishen Zheng

    Postdoctoral Researcher

    2007-2014

    lei liu tsinghua university

    Huan Lan

    Graduate Student

    2013-2016

    lei liu,tsinghua

    Weiliang Xu

    Postdoctoral Researcher

    2013-2016

    lei liu tsinghua university

    Demeng Sun

    Postdoctoral Researcher

    2013-2015

    lei liu tsinghua university

    Ruilong Xie

    Postdoctoral Researcher

    2014-2016

    lei liu, chemistry

    Haonan Chang

    Graduate Student

    2010-2015

    lei liu ,chemistry

    Ye Guo

    Graduate Student

    2010-2015

    lei liu ,chemistry

    Hongkui Cui

    Graduate Student

    2008-2013

    lei liu ,chemistry

    Bin Xiao

    Postdoctoral Researcher

    2011-2013

    lei liu ,chemistry

    Yiming Li

    Postdoctoral Researcher

    2009-2012

    lei liu ,chemistry

    Xuemei Zhang

    Postdoctoral Researcher

    2010-2012

    lei liu ,chemistry

    Fei Shen

    Graduate Student

    2007-2012

  • PUBLICATIONS

     

    Chemical Synthesis of Diubiquitin-Based Photoaffinity Probes for Selectively Profiling Ubiquitin-Binding Proteins

    Angew. Chem. Int. Ed. 2017, 56, 1 – 6

     

    Monomer/Oligomer Quasi-Racemic Protein Crystallography

    J. Am. Chem. Soc. 2016, 138 , 14497–14502

     

    Quasi-​racemic X-​ray structures of K27-​linked ubiquitin chains prepared by total chemical synthesis.

    J. Am. Chem. Soc. 2016, 138, 7429-7435.

     

    A synthetic molecular system capable of mirror-image genetic replication and transcription.

    Nat. Chem. 2016, 8, 698-704.

     

    Robust chemical synthesis of membrane proteins through a general method of removable backbone modification.

    J. Am. Chem. Soc. 2016, 138, 3553-3561.

     

    Total chemical synthesis of photoactivatable proteins for light-​controlled manipulation of antigen-​antibody interactions.

      Chem. Sci. 2016, 7, 1891-1895.

     

    Utilization of a photoactivatable antigen system to examine B-​cell probing termination and the B-​cell receptor sorting mechanisms during B-​cell activation.

    Proc. Natl. Acad. Sci. USA 2016, 113, E558-E567.

     

    Diaminodiacid bridges to improve folding and tune the bioactivity of disulfide-rich peptides.

    Angew. Chem. Int. Ed. 2015, 54, 14276-14281.

     

    Blocking of the PD-1/PD-L1 interaction by a D-peptide antagonist for cancer immunotherapy.

    Angew. Chem. Int. Ed. 2015, 54, 11760-11764.

     

    Chemical synthesis of a two-photon-activatable chemokine and photon-guided lymphocyte migration in vivo.

    Nat. Commun. 2015, 6, 7220.

     

    An efficient one-pot four-segment condensation method for protein chemical synthesis.

    Angew. Chem. Int. Ed. 2015, 54, 5713-5717.

     

    Peptide o-aminoanilides as crypto-thioesters for protein chemical synthesis.

    Angew. Chem. Int. Ed. 2015, 54, 2194-2198.

     

    Synthesis of and specific antibody generation for glycopeptides with arginine N-GlcNAcylation.

    Angew. Chem. Int. Ed. 2014, 53, 14517-14521.

     

    Expedient total synthesis of small to medium-sized membrane proteins via Fmoc chemistry.

    J. Am. Chem. Soc. 2014, 136, 3695-3704.

     

    Irreversible site-specific hydrazinolysis of proteins by use of sortase.

      Angew. Chem. Int. Ed. 2014, 53, 2198-2202.

     

    Chemical synthesis of proteins using peptide hydrazides as thioester surrogates.

    Nat. Protoc. 2013, 8, 2483-2495.

     

    Diaminodiacid-based solid-phase synthesis of peptide disulfide bond mimics.

    Angew. Chem. Int. Ed. 2013, 52, 9558 –9562

     

    Development of new thioester equivalents for protein chemical synthesis.

    Acc. Chem. Res. 2013, 46, 2475-2484.

     

    Synthesis of autophagosomal marker protein LC3-II under detergent-free conditions.

    Angew. Chem. Int. Ed. 2013, 52, 4858-4862.

     

    Convergent chemical synthesis of proteins by ligation of peptide hydrazides.

      Angew. Chem. Int. Ed. 2012, 51, 10347-10350.

     

    Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling.

    Chem. Sci. 2012, 3, 2766-2770.

     

    Fmoc synthesis of peptide thioesters without post-chain-assembly manipulation.

    J. Am. Chem. Soc. 2011, 133, 11080-11083.

     

    Protein chemical synthesis by ligation of peptide hydrazides.

      Angew. Chem. Int. Ed. 2011, 50, 7645-7649.

     

    The Old Gate of Tsinghua University

    The Old Gate is a symbol of Tsinghua University

  • CONTACT

    Beijing , Haidian District , Tsinghua University, Hetian Building

  • LINKS

    lei liu, Tsinghua

    Tsinghua University

    lei liu, Tsinghua

    Department of Chemistry

    lei liu, Tsinghua

    Tsinghua-Peking Center for Life Sciences

    lei liu, Tsinghua

    School of Medicine

    lei liu,Tsinghua

    School of Life Science