Research in our group focuses on chemical protein synthesis and its applications to biomedical studies. Students will have good opportunities to participate in interdisciplinary research in the area of protein chemical synthesis and its biomedical applications. Welcome to join us : )
Proteins are fascinating molecules of life, but not every protein can be obtained biologically. Synthetic chemistry provides a supplementary approach for the production of proteins. This method expands our exploration of chemistry of life and drug development. Our research projects include:
1995 ~ 1999： B.S. , University of Science and Technology of China
1999 ~ 2004： Ph.D. , Columbia University
(with Professor Ronald Breslow)
2004 ~ 2007： Research Associate, The Scripps Research Institute
(with Professor Chi-Huey Wong)
2007 ~ present： Professor of Chemistry, Tsinghua University
2014 ~ present： Vice Chair, Department of Chemistry
2015 ~ present：Associate Editor, Organic & Biomolecular Chemistry
Chemical Synthesis of Diubiquitin-Based Photoaffinity Probes for Selectively Profiling Ubiquitin-Binding Proteins
Monomer/Oligomer Quasi-Racemic Protein Crystallography
Quasi-racemic X-ray structures of K27-linked ubiquitin chains prepared by total chemical synthesis.
A synthetic molecular system capable of mirror-image genetic replication and transcription.
Robust chemical synthesis of membrane proteins through a general method of removable backbone modification.
Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions.
Utilization of a photoactivatable antigen system to examine B-cell probing termination and the B-cell receptor sorting mechanisms during B-cell activation.
Diaminodiacid bridges to improve folding and tune the bioactivity of disulfide-rich peptides.
Blocking of the PD-1/PD-L1 interaction by a D-peptide antagonist for cancer immunotherapy.
Chemical synthesis of a two-photon-activatable chemokine and photon-guided lymphocyte migration in vivo.
An efficient one-pot four-segment condensation method for protein chemical synthesis.
Peptide o-aminoanilides as crypto-thioesters for protein chemical synthesis.
Synthesis of and specific antibody generation for glycopeptides with arginine N-GlcNAcylation.
Expedient total synthesis of small to medium-sized membrane proteins via Fmoc chemistry.
Irreversible site-specific hydrazinolysis of proteins by use of sortase.
Chemical synthesis of proteins using peptide hydrazides as thioester surrogates.
Diaminodiacid-based solid-phase synthesis of peptide disulfide bond mimics.
Development of new thioester equivalents for protein chemical synthesis.
Synthesis of autophagosomal marker protein LC3-II under detergent-free conditions.
Convergent chemical synthesis of proteins by ligation of peptide hydrazides.
Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling.
Fmoc synthesis of peptide thioesters without post-chain-assembly manipulation.
Protein chemical synthesis by ligation of peptide hydrazides.
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